Widespread homologous recombination within and between Streptomyces species
نویسندگان
چکیده
منابع مشابه
Mutants of Streptomyces roseosporus that express enhanced recombination within partially homologous genes.
Streptomyces roseosporus mutants that express enhanced recombination between partially homologous (homeologous) sequences were isolated by selection for recombination between the bacteriophage phi C31 derivative KC570 containing the Streptomyces coelicolor glucose kinase (glk) gene and the S. roseosporus chromosome. The frequencies of homeologous recombination in the ehr mutants were determined...
متن کاملRecombination Between Existing Homologous Chromosomes
Enchanced survival of ultraviolet-irradiated Escherichia coli dependent upon the recA + gene appears to result from the ability to seal gaps in newly synthesized deoxyribonucleic acid (DNA) produced when DNA containing pyrimidine dimers is replicated (8). Strains which are recombination-deficient (recA) do not seal these gaps and degrade their DNA to a considerable extent. Rupp and Howard-Fland...
متن کاملGenetic Recombination in Crosses Between Streptomyces aureofaciens and Streptomyces rimosus.
Polsinelli, M. (University of Pavia, Pavia, Italy), and Maria Beretta. Genetic recombination in crosses between Streptomyces aureofaciens and Streptomyces rimosus. J. Bacteriol. 91:63-68. 1966.-Biochemical mutants were obtained from Streptomyces rimosus and S. aureofaciens by ultraviolet irradiation. Crosses were performed between auxotrophic strains of S. rimosus and S. aureofaciens with posit...
متن کاملInteractive competition between homologous recombination and non-homologous end joining.
DNA-dependent protein kinase (DNA-PK), composed of Ku70, Ku80, and the catalytic subunit (DNA-PKcs), is involved in double-strand break (DSB) repair by non-homologous end joining (NHEJ). DNA-PKcs defects confer ionizing radiation sensitivity and increase homologous recombination (HR). Increased HR is consistent with passive shunting of DSBs from NHEJ to HR. We therefore predicted that inhibitin...
متن کاملDesigning E1 Deleted Adenoviral Vector by Homologous Recombination
Adenoviruses are used extensively to deliver genes into mammalian cells, particularly where there is a requirement for high-level expression of transgene products in cultured cells, or for use as recombinant viral vaccines or in gene therapy. In spite of their usefulness, the construction of adenoviral vectors (AdV) is a cumbersome and lengthy process that is not readily amenable to the generat...
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ژورنال
عنوان ژورنال: The ISME Journal
سال: 2010
ISSN: 1751-7362,1751-7370
DOI: 10.1038/ismej.2010.45